Spermidine-condensed phi X174 DNA cleavage by micrococcal nuclease: torus cleavage model and evidence for unidirectional circumferential DNA wrapping.

@article{Marx1982SpermidinecondensedPX,
  title={Spermidine-condensed phi X174 DNA cleavage by micrococcal nuclease: torus cleavage model and evidence for unidirectional circumferential DNA wrapping.},
  author={Kenneth A. Marx and Thomas C. Reynolds},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1982},
  volume={79 21},
  pages={6484-8}
}
Spermidine-condensed phi X174 replicative form (RF) II DNA was digested with micrococcal nuclease to yield seven identifiable DNA bands forming an arithmetic fragment-length series. The DNA monomer unit length was found to be 780 +/- 80 base pairs. This result is most consistent with a proposed model for micrococcal nuclease cleavage of a DNA torus… CONTINUE READING