Spermatid injection into human oocytes. I. Laboratory techniques and special features of zygote development.

@article{Tesarik1996SpermatidII,
  title={Spermatid injection into human oocytes. I. Laboratory techniques and special features of zygote development.},
  author={Jan Tesarik and Carmen Mendoza},
  journal={Human reproduction},
  year={1996},
  volume={11 4},
  pages={
          772-9
        }
}
Spermatid injection into the oocyte cytoplasm has been shown recently to yield viable human embryos developing to term after transfer to the mother. This study provides details of the laboratory techniques related to round spermatid injection (ROSI) and elongated spermatid injection (ELSI) and focuses on some special features of zygote development associated with the use of these types of sperm precursor cells for fertilization. A spermatid-enriched fraction was obtained by centrifugation of… Expand
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Is there a future for spermatid injections?
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More intensive work is needed to improve the selection and handling of cells and to ascertain the genomic imprinting and gene expression necessary for embryonic development in microinjection of spermatids into oocytes. Expand
Pregnancy and birth after transfer of embryos that developed from single-nucleated zygotes obtained by injection of round spermatids into oocytes.
TLDR
Embryos derived from single-nucleated zygotes after spermatid conception can be viable and give rise to an ongoing clinical pregnancy and childbirth. Expand
Pregnancy by the tubal transfer of embryos developed after injection of round spermatids into oocyte cytoplasm of the cynomolgus monkey (Macaca fascicularis).
TLDR
The round spermatids of cynomolgus monkeys can be used as substitute gametes to support embryonic development at least to mid-gestation and for biological and genetic characterization of events following ROSI. Expand
Transfer at the blastocyst stage of embryos derived from testicular round spermatid injection.
TLDR
The results of this study indicate that the blastocyst stage is reached by only very few ROSI-derived embryos and these embryos do not implant. Expand
IS THERE A PLACE FOR ROUND AND ELONGATED SPERMATIDS INJECTION IN ASSISTED REPRODUCTION
TLDR
Up to date, it appears that the rates of fertilization and implantation with round spermatid injection are dramatically low and significantly less compared to the use of elongated sperMatid injection. Expand
Fourteen babies born after round spermatid injection into human oocytes
TLDR
All children born after ROSI in the clinic are without any unusual physical, mental, or epigenetic problems, and for men whose germ cells are unable to develop beyond the round spermatid stage, ROSI can enable them to have their own genetic offspring. Expand
Intracytoplasmic injection of spermatids retrieved from testicular tissue: influence of testicular pathology, type of selected spermatids and oocyte activation.
Spermatid microinjection into oocytes has proven to be a successful assisted reproduction procedure in the animal model and in the human species, since in the latter a few full-term pregnancies wereExpand
Functional assessment of centrosomes of spermatozoa and spermatids microinjected into rabbit oocytes
TLDR
Poor centrosomal function, as measured by diminished aster formation rates, is related to the poor fertilization rates when immature spermatogenic cells are injected. Expand
Successful fertilization and embryo development after spermatid injection: A hope for nonobstructive azoospermic patients
TLDR
The use of spermatids for intracytoplasmic injection (ICSI) has been proposed for cases in which no spermatozoa can be retrieved, however, there are low pregnancy rates following ICSI using round sPermatids from men with no elongated sperMatids or spermatoozoa in their testes. Expand
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TLDR
The results suggest that the method described here can be applied in a preliminary diagnostic test before a therapeutic SUZI attempt and in studies aimed at the optimization of sperm treatment protocols to increase the fusion capacity of subzonally inserted spermatozoa. Expand
Subzonal sperm insertion with aged human oocytes from failed in-vitro fertilization attempts: fertilization results and some applications.
TLDR
The results suggest that the method described here can be applied in a preliminary diagnostic test before a therapeutic SUZI attempt and in studies aimed at the optimization of sperm treatment protocols to increase the fusion capacity of subzonally inserted spermatozoa. Expand
Behaviour of hamster and mouse round spermatid nuclei incorporated into mature oocytes by electrofusion.
TLDR
Round spermatids of the hamster and mouse were electrofused with homologous mature oocytes to examine the behaviour of their nuclei within the ooplasm to find out whether nucleolus-like structures appeared de novo and DNA synthesis occurred in these small pronuclei. Expand
Mouse oocytes injected with testicular spermatozoa or round spermatids can develop into normal offspring.
TLDR
Under the experimental conditions employed, spermatid nuclei were less efficient than testicular sperm nuclei in producing normal offspring, but perhaps this was due to technical rather than inherent problems. Expand
Spermatid injection into human oocytes. II. Clinical application in the treatment of infertility due to non-obstructive azoospermia.
TLDR
Data show the intra-ooplasmic injection of spermatids obtained from the ejaculate may become the treatment of first choice in patients with non-obstructive azoospermia. Expand
Time-course of oocyte activation, pronucleus formation and cleavage in human oocytes fertilized by intracytoplasmic sperm injection.
TLDR
The morphological nuclear changes in 14 patients' oocytes were studied by means of light microscopic observation at 2, 4, 6, 8, 16, 18 and at 20 h after intracytoplasmic single sperm injection (ICSI). Expand
Round spermatid nuclei injected into hamster oocytes from pronuclei and participate in syngamy.
TLDR
Although the developmental potential of these zygotes could not be determined, spermatid nuclei appear capable of participating in syngamy. Expand
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To the Editor: By injecting sperm into the cytoplasm of an oocyte1 it is now possible to achieve fertilization and pregnancy in cases of obstructive azoospermia or severe oligozoospermia andExpand
Birth of normal young after electrofusion of mouse oocytes with round spermatids.
TLDR
It is demonstrated that the nuclei of spermatids can provide the paternally imprinted chromosomes needed for full embryonic development and also that the complex postmeiotic modifications involved in sperm formation in the testis and their maturation in the epididymis merely serve to facilitate natural delivery of the paternal genome. Expand
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TLDR
The process of human male pronuclear formation was studied using an experimental model based on in vitro inseminated human zona-free eggs prepared from oocytes that failed to fertilize in a clinical in vitro fertilization program to provide a morphological background for further research into molecular aspects of humanmale pronuclear development and its regulation. Expand
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