Sperm-zona binding and sperm-oocyte penetration analysed by DNA fluorescence: a test for gamete quality after in-vitro fertilization.


Sperm-zona binding, sperm-oocyte penetration and pronucleus formation in oocytes obtained after in-vitro fertilization (IVF) failure were studied by analysing DNA fluorescence. Oocytes were classified according to their maturational status, sperm-zona pellucida binding and sperm-oocyte penetration. Spermatozoa were classified as normal or abnormal according… (More)


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