Sperm telomere length in motile sperm selection techniques: A qFISH approach.

@article{Lafuente2018SpermTL,
  title={Sperm telomere length in motile sperm selection techniques: A qFISH approach.},
  author={Rafael Lafuente and E Bosch-Rue and Jordi Ribas-Maynou and J. {\'A}. Garijo {\'A}lvarez and C Brassesco and Mar{\'i}a Jos{\'e} Amengual and Jordi Benet and A Garcia-Peir{\'o} and Mario Brassesco},
  journal={Andrologia},
  year={2018},
  volume={50 2}
}
Several studies have associated telomere shortening with alterations in reproductive function. The objective of the present study was to determine telomere length (TL) in spermatozoa selected by either density-gradient centrifugation (DGC) or swim-up. The analysis of TL was performed using quantitative fluorescent in situ hybridisation (qFISH) using PNA probes in combination with a chromatin decompaction protocol in sperm cells. Results of TL were 24.64 ± 5.00 Kb and 24.95 ± 4.60 Kb before and… CONTINUE READING