Sperm membrane potential: hyperpolarization during capacitation regulates zona pellucida-dependent acrosomal secretion.

@article{Zeng1995SpermMP,
  title={Sperm membrane potential: hyperpolarization during capacitation regulates zona pellucida-dependent acrosomal secretion.},
  author={Yang Zeng and E N Clark and Harvey M Florman},
  journal={Developmental biology},
  year={1995},
  volume={171 2},
  pages={554-63}
}
Membrane potential (VM) was investigated in mouse and bovine sperm populations. VM was determined from the fluorescence emission of the lipophilic anion, bis(1,3-diethylthiobarbituric acid)trimethine oxonol (DiSBAC2(3)), and from the lipophilic cation, 3,3'-dipropylthiodicarbocyanine iodide (DiSC3(5)). Fluorescent signals were corrected for contributions of mitochondrial potentials and apparent VM values were obtained by calibrations in sperm selectively permeabilized with valinomycin or with… CONTINUE READING

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