Sperm-mediated gene transfer.

  title={Sperm-mediated gene transfer.},
  author={Marialuisa Lavitrano and Marco Busnelli and Maria Grazia Cerrito and Roberto Giovannoni and Stefano Manzini and Alessia Vargiolu},
  journal={Reproduction, fertility, and development},
  volume={18 1-2},
Since 1989, a new method for the production of transgenic animals has been available, namely sperm-mediated gene transfer (SMGT), based on the intrinsic ability of sperm cells to bind and internalise exogenous DNA molecules and to transfer them into the oocyte at fertilisation. We first described the SMGT procedure in a small animal model, with high efficiency reported in the mouse. In addition, we successfully adapted and optimised the technique for use in large animals; it was, in fact… CONTINUE READING


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