Sperm cryopreservation with empty human or mouse zona pellucidae. A comparison.

@article{Hsieh2000SpermCW,
  title={Sperm cryopreservation with empty human or mouse zona pellucidae. A comparison.},
  author={Yen Kung Hsieh and Horng Der Tsai and Chi Chen Chang and Hui Yu Lo},
  journal={The Journal of reproductive medicine},
  year={2000},
  volume={45 5},
  pages={
          383-6
        }
}
OBJECTIVE To compare empty zona pellucidae (ZP) and human of mouse oocytes for sperm cryopreservation. STUDY DESIGN ZP was prepared by evacuating the cytoplasm of human or mouse oocytes. The evacuated ZP were injected with spermatozoa from patients with severe oligoasthenozoospermia from normally fertile men. After a freezing-thawing procedure, the spermatozoa were aspirated outside the ZP. According to the different origins of ZP and sperm, the spermatozoa number per ZP, motile sperm number… CONTINUE READING

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