Sperm concentration influences fertilization and male pronuclear formation in vitro in pigs.

@article{Coy1993SpermCI,
  title={Sperm concentration influences fertilization and male pronuclear formation in vitro in pigs.},
  author={Pilar Coy and Eugenio Mart{\'i}nez and Salvador Ruiz and Juan Mar{\'i}a V{\'a}zquez and Jordi Roca and Carmen Mat{\'a}s},
  journal={Theriogenology},
  year={1993},
  volume={40 3},
  pages={539-46}
}
To study the effect of sperm concentration on the results of pig in vitro fertilization (IVF), 313 oocytes recovered from oviducts of prepubertal gilts after induction of ovulation were used. After capacitation, the number of live spermatozoa in the fertilization dishes was adjusted to 3 x 10(5), 6 x 10(5) and 12 x 10(5) cell/ml. After 4 hours of co-culture in TCM-199, the oocytes were pippeted to remove cumulus cells and the excess spermatozoa around the zona pellucida, and were transferred to… CONTINUE READING

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