Sperm chromatin anomalies can influence decondensation after intracytoplasmic sperm injection.

@article{Sakkas1996SpermCA,
  title={Sperm chromatin anomalies can influence decondensation after intracytoplasmic sperm injection.},
  author={Denny Sakkas and Françoise Urner and Patricia Grace Bianchi and Davide Bizzaro and Inken Wagner and N Jaquenoud and Gian Carlo Manicardi and A de P Campana},
  journal={Human reproduction},
  year={1996},
  volume={11 4},
  pages={837-43}
}
In this study we investigated whether morphology and chromatin anomalies in human spermatozoa can influence fertilization after intracytoplasmic sperm injection (ICSI). We examined unfertilized oocytes, using the fluorochrome Hoechst 33342, to determine whether a relationship exists between failure of fertilization and sperm chromatin quality. Sperm chromatin packaging quality was assessed using the chromomycin A3 (CMA3) fluorochrome, and the presence of DNA damage in spermatozoa, using in-situ… CONTINUE READING