Sperm arylsulfatase A binds to mZP2 and mZP3 glycoproteins in a nonenzymatic manner.

@article{Xu2012SpermAA,
  title={Sperm arylsulfatase A binds to mZP2 and mZP3 glycoproteins in a nonenzymatic manner.},
  author={Hongbin Xu and Fang Liu and Nopparat Srakaew and Chaitanya A. K. Koppisetty and Per-Georg Nyholm and Eur{\'i}dice Carmona and Nongnuj Tanphaichitr},
  journal={Reproduction},
  year={2012},
  volume={144 2},
  pages={
          209-19
        }
}
We have shown previously that sperm surface arylsulfatase A (ASA) of mouse, pig, and human is involved in sperm-egg zona pellucida (ZP) binding. By treating capacitated mouse sperm with A23187 to induce the acrosome reaction, we demonstrated by immunoblotting that ASA also existed in the acrosomal content and on the inner acrosomal membrane. Since mZP2 and mZP3 are known as sperm receptors, whereas mZP1 as a cross-linker of mZP2/mZP3, we determined whether purified ASA bound to mZP2 and mZP3… CONTINUE READING
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