Sperm DNA damage measured by the alkaline Comet assay as an independent predictor of male infertility and in vitro fertilization success.

@article{Simon2011SpermDD,
  title={Sperm DNA damage measured by the alkaline Comet assay as an independent predictor of male infertility and in vitro fertilization success.},
  author={Luke Simon and Deborah Lutton and Joanne McManus and Sheena E. M. Lewis},
  journal={Fertility and sterility},
  year={2011},
  volume={95 2},
  pages={652-7}
}
OBJECTIVE To evaluate sperm DNA fragmentation and semen parameters to diagnose male factor infertility and predict pregnancy after IVF. DESIGN Prospective study. SETTING Academic research laboratory. PATIENT(S) Seventy-five couples undergoing IVF and 28 fertile donors. INTERVENTION(S) Sperm DNA fragmentation was measured by the alkaline Comet assay in semen and sperm after density gradient centrifugation (DGC). Binary logistic regression was used to analyze odds ratios (OR) and relative… CONTINUE READING
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