Spectroscopic selection of distance measurements in a protein dimer with mixed nitroxide and Gd3+ spin labels.

@article{Kaminker2012SpectroscopicSO,
  title={Spectroscopic selection of distance measurements in a protein dimer with mixed nitroxide and Gd3+ spin labels.},
  author={I. Kaminker and H. Yagi and T. Huber and A. Feintuch and G. Otting and D. Goldfarb},
  journal={Physical chemistry chemical physics : PCCP},
  year={2012},
  volume={14 13},
  pages={
          4355-8
        }
}
The pulse DEER (Double Electron-Electron Resonance) technique is frequently applied for measuring nanometer distances between specific sites in biological macromolecules. In this work we extend the applicability of this method to high field distance measurements in a protein assembly with mixed spin labels, i.e. a nitroxide spin label and a Gd(3+) tag. We demonstrate the possibility of spectroscopic selection of distance distributions between two nitroxide spin labels, a nitroxide spin label… Expand
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