Spectroscopic evidence for a porphobilinogen deaminase-tetrapyrrole complex that is an intermediate in the biosynthesis of uroporphyrinogen III.

@article{Ros1988SpectroscopicEF,
  title={Spectroscopic evidence for a porphobilinogen deaminase-tetrapyrrole complex that is an intermediate in the biosynthesis of uroporphyrinogen III.},
  author={S Ros{\'e} and ROSALIA B. Frydman and Carlos R. de los Santos and Adriana R. Sburlati and Aldonia Valasinas and Benjamin J Frydman},
  journal={Biochemistry},
  year={1988},
  volume={27 13},
  pages={
          4871-9
        }
}
Incubation of porphobilinogen (PBG) with PBG deaminase from Rhodopseudomonas sphaeroides in carbonate buffer (pH 9.2) to total PBG consumption resulted in low yields of uroporphyrinogen I (uro'gen I). In the reaction mixture a pyrrylmethane accumulated, which at longer incubation periods was transformed into uro'gen I. The accumulated pyrrylmethane gave an Ehrlich reaction which was different from that of a 2-(aminomethyl)dipyrrylmethane or 2-(aminomethyl)tripyrrane. It resembled that of a… Expand
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TLDR
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TLDR
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