Spectroscopic and structural study of proton and halide ion cooperative binding to gfp.

@article{Arosio2007SpectroscopicAS,
  title={Spectroscopic and structural study of proton and halide ion cooperative binding to gfp.},
  author={Daniele Arosio and Gianpiero Garau and Fernanda Ricci and Laura Marchetti and Ranieri Bizzarri and Riccardo Nifos{\'i} and Fabio Beltram},
  journal={Biophysical journal},
  year={2007},
  volume={93 1},
  pages={232-44}
}
This study reports the influence of halogens on fluorescence properties of the Aequorea victoria Green Fluorescent Protein variant S65T/T203Y (E(2)GFP). Halide binding forms a specific nonfluorescent complex generating a substantial drop of the fluorescence via static quenching. Spectroscopic analysis under different solution conditions reveals high halogen affinity, which is strongly dependent on the pH. This evidences the presence in E(2)GFP of interacting binding sites for halide ions and… CONTINUE READING