Spectroscopic and kinetic characterization of the recombinant wild-type and C242S mutant of the cytochrome b reductase fragment of nitrate reductase.

@article{Ratnam1995SpectroscopicAK,
  title={Spectroscopic and kinetic characterization of the recombinant wild-type and C242S mutant of the cytochrome b reductase fragment of nitrate reductase.},
  author={Karunaharan Ratnam and Naomasa Shiraishi and Wilbur H. Campbell and R. F. Hille},
  journal={The Journal of biological chemistry},
  year={1995},
  volume={270 41},
  pages={24067-72}
}
Spectroscopic and kinetic studies comparing the behavior of the recombinant cytochrome b reductase fragment of corn leaf nitrate reductase and a mutant in which cysteine 242 is replaced with a serine residue (C242S) have been carried out. The visible and circular dichroism spectra of the wild-type and mutant protein are virtually identical and compare well with those reported for nitrate reductases from other sources. The reduced wild-type protein forms a charge-transfer complex with NAD+ that… CONTINUE READING