Spectropolarimetric analysis of the core histone octamer and its subunits.

  title={Spectropolarimetric analysis of the core histone octamer and its subunits.},
  author={Jean Godfrey and Alexandros Baxevanis and Evangelos N. Moudrianakis},
  volume={29 4},
The secondary structure of the calf thymus core histone octamer, (H2A-H2B-H3-H4)2, and its two physiological subunits, the H2A-H2B dimer and (H3-H4)2 tetramer, was analyzed by ORD spectropolarimetry as a function of temperature and solvent ionic strength within the ranges of these experimental parameters where assembly of the core histone octamer exhibits pronounced sensitivity. While the secondary structure of the dimer is relatively stable from 0.1 to 2.0 M NaCl, the secondary structure of… CONTINUE READING