Spectrophotometric quantitation of antioxidant capacity through the formation of a phosphomolybdenum complex: specific application to the determination of vitamin E.

@article{Prieto1999SpectrophotometricQO,
  title={Spectrophotometric quantitation of antioxidant capacity through the formation of a phosphomolybdenum complex: specific application to the determination of vitamin E.},
  author={Paloma Prieto and Manuel A. Pineda and Marie-isabel Aguilar},
  journal={Analytical biochemistry},
  year={1999},
  volume={269 2},
  pages={337-41}
}
A spectrophotometric method has been developed for the quantitative determination of antioxidant capacity. The assay is based on the reduction of Mo(VI) to Mo(V) by the sample analyte and the subsequent formation of a green phosphate/Mo(V) complex at acidic pH. The method has been optimized and characterized with respect to linearity interval, repetitivity and reproducibility, and molar absorption coefficients for the quantitation of several antioxidants, including vitamin E. The… CONTINUE READING
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