Corpus ID: 38792903

Spectrophotometric analysis of total ascorbic acid contetnt in various fruits and vegetables

  title={Spectrophotometric analysis of total ascorbic acid contetnt in various fruits and vegetables},
  author={{\vC}opra and Jani{\'c}ijevi{\'c}},
Total ascorbic acid (ascorbic acid + dehydroascorbic acid) has been determined in twenty-one different samples of fruits and vegetables by spectrophotometric method. This method is based on the oxidation ascorbic acid to dehydroascorbic acid by bromine water in the presence of acetic acid. After coupling with 2,4-dinitrophenylhydrazine (DNPH) a red complex was produced and absorbance of that complex was spectrophotometrically measured at 521 nm. A linear concentration range for standard… Expand

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