Spectrophotometric Simultaneous Analysis of Ambroxol Hydrochloride , Guaifenesin and Terbutaline Sulphate in Liquid Dosage Form ( Syrup )

Abstract

This study proposes a method for simultaneous estimation of Ambroxol HCl, Guaifenesin and Terbutaline sulphate in syrup form. The study was done by combining three spectrophotometric methods viz use of specific absorbance [A 1%, 1cm], second order derivative and colorimetry. Absorption of Guaifenesin and Terbutaline sulphate were found to be zero at 307.5nm, thus enabling the measurement of Ambroxol HCl, using specific absorbance in zero order spectrum. Applying the second order derivative, the amplitude of Guaifenesin was measured at 279.4nm, while Ambroxol HCl and Terbutaline sulphate were at zero cross point. For colorimetric measurement of Terbutaline Sulphate, a colored substance was obtained by coupling the oxidized product of Terbutaline sulphate with 4-aminoantipyrine and its absorption was measured at 550nm. The proposed method was statistically validated in accordance with ICH guidelines and results were found to be satisfactory for accuracy, precision and specificity.

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Cite this paper

@inproceedings{Kimbahune2011SpectrophotometricSA, title={Spectrophotometric Simultaneous Analysis of Ambroxol Hydrochloride , Guaifenesin and Terbutaline Sulphate in Liquid Dosage Form ( Syrup )}, author={Ritu Kimbahune and Sunil K and Prachi Kabra and Kuldeep Delvadiya and Sanjay Surani}, year={2011} }