Spectrophotometric Determination of Azathioprine in Bulk and Pharmaceutical Dosage Forms

Abstract

Two simple, accurate and precise Spectrophotometric methods have been developed for the determination of Azathioprine in bulk and pharmaceutical dosage forms.Method A is based on oxidation followed by complexation reaction of reduced Azathioprine with potassium ferricyanide in presence of ferric chloride to form a greenish blue coloured chromogen with an absorption maximum at 667.5nm and Beer’s law is obeyed in the concentration range of 60-120 mg /ml. Method B is based on the diazotization of reduced Azathioprine in presence of nitric acid and followed by direct coupling with Bratton Marshall reagent to form dark pink colored chromogen with an absorption maximum at 519nm and obeyed Beer’s law in the concentration range of 20-50 μg/ml.

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Cite this paper

@inproceedings{Bhaskar2003SpectrophotometricDO, title={Spectrophotometric Determination of Azathioprine in Bulk and Pharmaceutical Dosage Forms}, author={Malayappan Bhaskar and Y. N. Manohara and MD . GAYASUDDIN MOUID and M . BALARAJU and T. Bharath}, year={2003} }