Spectrofluorometric method for the determination of ascorbic acid in pharmaceutical preparation using l-tyrosine as fluorescence probe.

  title={Spectrofluorometric method for the determination of ascorbic acid in pharmaceutical preparation using l-tyrosine as fluorescence probe.},
  author={Yuting Wu and Qinfei Chen and Lijun Zhao and Dong Du and Nihong Guo and Haisheng Ren and Wenbin Liu},
  journal={Luminescence : the journal of biological and chemical luminescence},
Ascorbic acid is a vital nutrient and antioxidant that is commonly used as an additive in commercial products. Quantitation of ascorbic acid is highly desired in the medical, food, and cosmetic industries. A spectrofluorometric assay for sensitive determination of ascorbic acid was developed using l-tyrosine as a fluorescent probe. The native fluorescence intensity of tyrosine was quenched using ascorbic acid. The linear range was 0.03-30.00 μM, and the limit of detection was 0.01 μM. The… Expand
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