Spectrofluorimetric quantification of malondialdehyde for evaluation of cyclooxygenase-1/thromboxane synthase inhibition.

@article{Dannhardt1998SpectrofluorimetricQO,
  title={Spectrofluorimetric quantification of malondialdehyde for evaluation of cyclooxygenase-1/thromboxane synthase inhibition.},
  author={Gerd Dannhardt and L Flemmer and Rolf W. Hartmann and Astrid Kleber and E. Schulze},
  journal={Archiv der Pharmazie},
  year={1998},
  volume={331 11},
  pages={359-64}
}
The in vitro assay developed by Hartmann and Ledergerber (1995) utilizing the spectrofluorimetric quantification of malondialdehyde after reaction with thiobarbituric acid was modified and used for further investigations. The human whole blood was replaced by a platelet suspension of pig blood, and calcium ionophore A23187 was used instead of collagen for inducing the arachidonic acid cascade. The modified assay represents a simple, time and cost saving method for the evaluation of… CONTINUE READING