Spectrally resolved fluorescence lifetime imaging microscopy (SFLIM)--an appropriate method for imaging single molecules in living cells.

Abstract

Ever since the first examination of cellular structures, scientists have been fascinated by investigations of single cells. Over the last decade, many methods (e.g., capillary electrophoresis, electrochemical detection, mass spectroscopy, and optical spectroscopy) have been proposed for analyzing single cells [1]. Many of these methods are appropriate for… (More)

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