Spectral tuning of photoproteins by partnering site-directed mutagenesis strategies with the incorporation of chromophore analogs.

@article{Rowe2008SpectralTO,
  title={Spectral tuning of photoproteins by partnering site-directed mutagenesis strategies with the incorporation of chromophore analogs.},
  author={Laura Rowe and Anna Rothert and C J Logue and Charles Mark Ensor and Sapna K Deo and Sylvia Daunert},
  journal={Protein engineering, design & selection : PEDS},
  year={2008},
  volume={21 2},
  pages={73-81}
}
Aequorin and obelin are photoproteins whose calcium controlled bioluminescent light emission is used for labeling in assays, for the determination of calcium concentrations in vivo, and as a reporter in cellular imaging. Both of these photoproteins emit blue light from a 2-hydroperoxycoelenterazine chromophore, which is non-covalently bound in the hydrophobic core of the proteins. In an effort to produce aequorin and obelin variants with improved analytical properties, such as alternative… CONTINUE READING

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Edited by Andreas Kungl Spectral tuning of photoproteins by partnering site-directed mutagenesis strategies

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