Spectral properties of the prototropic forms of fluorescein in aqueous solution

@article{Klonis1996SpectralPO,
  title={Spectral properties of the prototropic forms of fluorescein in aqueous solution},
  author={Nectarios Klonis and William H. Sawyer},
  journal={Journal of Fluorescence},
  year={1996},
  volume={6},
  pages={147-157}
}
The commonly used fluorescent probe, fluorescein, can exist in seven prototropic forms. We have used global analysis procedures to reanalyze the absorption data of Diehl and Horchak-Morris (Talanta 34, 739–741, 1987) in terms of five alternative ionization models. We identify the forms of fluorescein present in aqueous solution and the pK a of each ionisation transition. The pKa values of the neutral xanthene, carboxylic acid, and cationic xanthene groups are 6.3, 3.1–3.4, and 3.1–3.4… CONTINUE READING

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