Spectral and kinetic properties of the Fet3 protein from Saccharomyces cerevisiae, a multinuclear copper ferroxidase enzyme.

@article{Hassett1998SpectralAK,
  title={Spectral and kinetic properties of the Fet3 protein from Saccharomyces cerevisiae, a multinuclear copper ferroxidase enzyme.},
  author={Richard Hassett and Daniel S. Yuan and Daniel J Kosman},
  journal={The Journal of biological chemistry},
  year={1998},
  volume={273 36},
  pages={
          23274-82
        }
}

• Richard Hassett, Daniel S. Yuan, Daniel J Kosman
• Published in The Journal of biological chemistry 1998

High affinity iron uptake in Saccharomyces cerevisiae requires Fet3p. Fet3p is proposed to facilitate iron uptake by catalyzing the oxidation of Fe(II) to Fe(III) by O2; in this model, Fe(III) is the substrate for the iron permease, encoded by FTR1. Here, a recombinant Fet3p has been produced in yeast that, lacking the C-terminal membrane-spanning domain, is secreted directly into the growth medium. Solutions of this Fet3p at >1 mg/ml have the characteristic blue color of a type 1 Cu(II… CONTINUE READING

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