Spectral analysis for base composition of DNA undergoing melting.

Abstract

A microcomputer-controlled spectrophotometer is described for obtaining the base composition of melting domains in DNA from derivative melting curves. Values have been determined for the differential molar extinction coefficients for the A-T and G-C base pair at the three wavelengths most useful for spectral analysis of base composition, 260, 270 and 282 nm… (More)

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Cite this paper

@article{Blake1985SpectralAF, title={Spectral analysis for base composition of DNA undergoing melting.}, author={Richard D. Blake and T G Hydorn}, journal={Journal of biochemical and biophysical methods}, year={1985}, volume={11 6}, pages={307-16} }