Spectral Self-Interference Fluorescence Microscopy for Subcellular Imaging

@article{Doan2008SpectralSF,
  title={Spectral Self-Interference Fluorescence Microscopy for Subcellular Imaging},
  author={Mehmet Doğan and A. Yalcn and Samiksha Jain and M Goldberg and A. P. Swan and M. Unlu and B. Goldberg},
  journal={IEEE Journal of Selected Topics in Quantum Electronics},
  year={2008},
  volume={14},
  pages={217-225}
}
Spectral self-interference fluorescence microscopy (SSFM) has been recently developed to determine the axial position of fluorescent emitters placed on reflecting dielectric structures. In this paper, we review SSFM with emphasis on its axial localization capabilities. We show that there is a tradeoff between the numerical aperture (NA) of the microscopy system and the axial localization fidelity. To use high-NA objectives for better lateral resolution, we describe and demonstrate a high-NA 4Pi… CONTINUE READING

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