Spectra of cells in flow cytometry using a vidicon detector.

  title={Spectra of cells in flow cytometry using a vidicon detector.},
  author={Chuck Wade and R H Rhyne and William H. Woodruff and David P. Bloch and James C. Bartholomew},
  journal={The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society},
  volume={27 6},
A TV type vidicon detector was interfaced to a flow cytometer (FCM) to obtain spectra of fluorophores in cells during flow. The normal operations of the FCM are undisturbed. A spectrograph spreads 320 nm of the fluorophore fluorescence emission across the 500 channels of the detector. Spectra of fluorescamine (a surface labeling agent) and of propidium iodide (a nuclear stain) were obtained from Balb 3T3 cells, and the chlorophyll and phycobilin peaks were resolved from flowing blue-green algae… CONTINUE READING


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