Spectinomycin resistance at site 1192 in 16S ribosomal RNA of E. coli: an analysis of three mutants.


Three different single base substitutions were constructed at residue C-1192 in 16S rRNA on a plasmid-coded rrnB operon of E. coli using site-directed mutagenesis. All 3 mutants conferred different levels of resistance to spectinomycin in transformed cells but none affected the growth rate in the absence of the antibiotic. The G-1192 mutant conferred… (More)


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