Specificity of radioprotective and cytotoxic effects of cysteamine in HeLa S3 cells: generation of peroxide as the mechanism of paradoxical toxicity.

@article{Takagi1974SpecificityOR,
  title={Specificity of radioprotective and cytotoxic effects of cysteamine in HeLa S3 cells: generation of peroxide as the mechanism of paradoxical toxicity.},
  author={Y. Takagi and M. Shikita and T. Terasima and S. Akaboshi},
  journal={Radiation research},
  year={1974},
  volume={60 2},
  pages={
          292-301
        }
}
  • Y. Takagi, M. Shikita, +1 author S. Akaboshi
  • Published 1974
  • Chemistry, Medicine
  • Radiation research
  • Cysteamine at 0.5-5 mM effectively kills ${\rm HeLa}\ {\rm S}_{3}$ cells, while it is much less toxic in higher concentration (30 mM). The toxicity develops gradually with time as peroxide generates in the medium. Addition of catalase and peroxidase to the culture inhibits the toxic effect of cysteamine. The results suggest that the paradoxical cell-killing action of cysteamine can be ascribed to the generation of the peroxide by the compound ( $2{\rm RSH}+{\rm O}_{2}={\rm RSSR}+{\rm H}_{2}{\rm… CONTINUE READING
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