Specificity of nonadrenergic imidazoline binding sites in insulin-secreting cells and relation to the block of ATP-sensitive K(+) channels.

@article{GrosseLackmann2003SpecificityON,
  title={Specificity of nonadrenergic imidazoline binding sites in insulin-secreting cells and relation to the block of ATP-sensitive K(+) channels.},
  author={Timm Grosse-Lackmann and Bernd J Zunkler and Ingo Rustenbeck},
  journal={Annals of the New York Academy of Sciences},
  year={2003},
  volume={1009},
  pages={371-7}
}
To characterize the specificity of nonadrenergic imidazoline binding sites of insulin-secreting HIT cells, competitive binding of insulinotropic imidazolines and quinine was measured and compared with the effect of these compounds on native K(ATP) channels and with a heterologously expressed variant of the pore-forming subunit (Kir6.2 deltaC26). There were two nonadrenergic imidazoline binding sites for [(3)H]clonidine with K(d) values of 61 nM and 4.5 microM, respectively. Quinine reduced… CONTINUE READING