Specificity of intrathecal IgG synthesis for HTLV-1 core and envelope proteins in HAM/TSP.


INTRODUCTION In patients with human T-cell lymphotropic virus type 1 (HTLV-1) associated myelopathy/tropical spastic paraparesis (HAM/TSP), we investigated the significance of HTLV-1 specific antibodies in the cerebrospinal fluid (CSF). MATERIAL AND METHODS The quantities of HTLV-1 specific immunoglobulin G (IgG) in paired CSF and serum were evaluated by a sensitive enzyme immunoassay (EIA). The specificity of antiviral IgG was determined by radioimmunoprecipitation of HTLV-1 antigens. RESULTS In 17 of 20 HAM/TSP patients, quantitative evaluation by EIA supplied evidence for antiviral IgG synthesis within the CNS. Radioimmunoprecipitation demonstrated IgG antibodies against HTLV-1 envelope and core proteins in all HAM/TSP CSF and sera tested. Regarding the 3 sample pairs indeterminate in EIA for intrathecal synthesis, 2 showed stronger precipitation of HTLV-1 antigens by CSF IgG than by equal amounts of serum IgG. CONCLUSION Intrathecal antibody synthesis specific for both HTLV-1 core and envelope antigens is common in HAM/TSP, thus providing conclusive evidence for an immune response to HTLV-1 within the CNS.

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@article{Kitze1995SpecificityOI, title={Specificity of intrathecal IgG synthesis for HTLV-1 core and envelope proteins in HAM/TSP.}, author={Bernd Kitze and Marzia Puccioni-Sohler and J W Sch{\"a}ffner and Peter Rieckmann and Thomas Weber and Klaus Felgenhauer and Walter Bodemer}, journal={Acta neurologica Scandinavica}, year={1995}, volume={92 3}, pages={213-7} }