Specificity of glucocorticoid receptor primary antibodies for analysis of receptor localization patterns in cultured cells and rat hippocampus.

@article{Sarabdjitsingh2010SpecificityOG,
  title={Specificity of glucocorticoid receptor primary antibodies for analysis of receptor localization patterns in cultured cells and rat hippocampus.},
  author={Ratna Angela Sarabdjitsingh and Onno C. Meijer and E. Ron de Kloet},
  journal={Brain research},
  year={2010},
  volume={1331},
  pages={1-11}
}
After glucocorticoid stimulation, glucocorticoid receptors (GRs) are translocated to the nucleus to modulate transcription of glucocorticoid target genes. The subcellular distribution and trafficking of GR in cultured cells has been studied quite intensively using several techniques. However, the intracellular localization of nuclear receptors in ligand-free and stimulated conditions in vivo is still controversial, in part because of inconsistent results with different antibodies. Knowledge of… CONTINUE READING
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