Specificity of elongation factor EF-TU for hydrophobic peptides.

  title={Specificity of elongation factor EF-TU for hydrophobic peptides.},
  author={Abdelharim Malki and Teresa D Caldas and Andrea Parmeggiani and Masamichi Kohiyama and Gilbert Richarme},
  journal={Biochemical and biophysical research communications},
  volume={296 3},
The elongation factor EF-Tu carries aminoacyl-tRNAs to the A-site of the ribosome during the elongation process of protein biosynthesis. We, and others, have recently reported that the Escherichia coli EF-Tu interacts with unfolded and denatured proteins and behaves like a chaperone in protein folding and protection against protein thermal denaturation. In this study, we have identified EF-Tu binding sites in protein substrates by screening cellulose-bound peptides scanning the sequences of… CONTINUE READING