Specificity of DnaK-peptide binding.

@article{Gragerov1994SpecificityOD,
  title={Specificity of DnaK-peptide binding.},
  author={Alexander Gragerov and Li Zeng and Xin Zhao and William F. Burkholder and Max E. Gottesman},
  journal={Journal of molecular biology},
  year={1994},
  volume={235 3},
  pages={848-54}
}
The sequence specificity of DnaK substrate binding has been studied using a peptide display library. Based on the amino acid patterns that appeared in this selection, short peptides were synthesized for direct measurements of DnaK affinity. The results show that peptides enriched in internal hydrophobic residues are preferential DnaK substrates, and negatively charged peptides have poor affinity. The isolated C-terminal domain of DnaK binds peptides. Peptide dissociation studies indicate that… CONTINUE READING

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