Specificity improvement of a recombinant anti-testosterone Fab fragment by CDRIII mutagenesis and phage display selection.

@article{Hemminki1998SpecificityIO,
  title={Specificity improvement of a recombinant anti-testosterone Fab fragment by CDRIII mutagenesis and phage display selection.},
  author={Ari Hemminki and Seija Niemi and A M Hoffr{\'e}n and Leena Hakalahti and Hans S{\"o}derlund and Kristiina Takkinen},
  journal={Protein engineering},
  year={1998},
  volume={11 4},
  pages={311-9}
}
The monoclonal antibodies so far developed by hybridoma technology have not had high enough specificity or affinity to distinguish the closely related steroid hormones in routine clinical assays. We have employed random mutagenesis and phage display approaches to improve the specificity of one anti-testosterone monoclonal antibody (3-C4F5). The affinity of the antibody is 0.3 x 10(9) M(-1) and the cross-reactivities with most of the related steroids are low. However, the antibody cross-reacts… CONTINUE READING

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