Specific techniques for the identification of O-acylated sialic acids in colonic mucins.

@article{Culling1980SpecificTF,
  title={Specific techniques for the identification of O-acylated sialic acids in colonic mucins.},
  author={C. F. A. Culling and Philip E. Reid},
  journal={Journal of microscopy},
  year={1980},
  volume={119 3},
  pages={415-25}
}
The development of methodology for the histochemistry of mucins based upon their PAS reactivity is discussed in terms of mechanism, specificity and application. Two new histochemical methods (PB/KOH/PAS and PAT/KOH/PAS), supplemented by a variety of new and standard histochemical techniques, and correlated by parallel chemical studies, were used to demonstrate and identify C4 and side chain O-acylated sialic acids in colonic epithelial mucins. The application of these methods in the field of… CONTINUE READING

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