Specific secretion of active single-chain Fv antibodies into the supernatants of Escherichia coli cultures by use of the hemolysin system.

@article{Fernndez2000SpecificSO,
  title={Specific secretion of active single-chain Fv antibodies into the supernatants of Escherichia coli cultures by use of the hemolysin system.},
  author={Luis {\'A}ngel Fern{\'a}ndez and Isabel Sola and Luis Enjuanes and V{\'i}ctor de Lorenzo},
  journal={Applied and environmental microbiology},
  year={2000},
  volume={66 11},
  pages={5024-9}
}
A simple method for the nontoxic, specific, and efficient secretion of active single-chain Fv antibodies (scFvs) into the supernatants of Escherichia coli cultures is reported. The method is based on the well-characterized hemolysin transport system (Hly) of E. coli that specifically secretes the target protein from the bacterial cytoplasm into the extracellular medium without a periplasmic intermediate. The culture media that accumulate these Hly-secreted scFv's can be used in a variety of… CONTINUE READING

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