Specific recognition of DNA bulge sites by in situ grown fluorescent Ag nanoclusters with high selectivity.

@article{Peng2014SpecificRO,
  title={Specific recognition of DNA bulge sites by in situ grown fluorescent Ag nanoclusters with high selectivity.},
  author={Jian Peng and Yong Shao and Lingling Liu and L. Zhang and Hua Liu},
  journal={Dalton transactions},
  year={2014},
  volume={43 4},
  pages={
          1534-41
        }
}
There is much demand for the development of reliable methods of bulge site recognition in DNA in order to find disease-related mutations and identify bulge sequence environments. Herein, we found that the bulge site in the DNA duplex can be used to selectively grow fluorescent silver nanoclusters (Ag NCs). The as-prepared Ag NCs exhibit an emission behavior dependent on the base environment near the bulge site and can be employed in situ as a readout for specific bulge site recognition… 
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8 Citations

8 Citations

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Citation Type

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This review systematically presents a wide-range of DNA-AgNCs based on the structural complexity of the DNA templates, including single-stranded DNA (ssDNA), double-strander DNA (dsDNA), triple-stranding DNA (tsDNA) and DNA nanostructures, and discusses the emission property, quantum yield and synthesis condition of the respective AgNCs.
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The results demonstrate that the target cellular fluorescence bio-imaging can readily realize through the in situ biosynthesis of biocompatible zinc nanoclusters from cancerous cells when target cells are cultured with micromolar zinc gluconate solutions.
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In this review, a broad overview of MNC design strategies and biomedical applications is provided and the urgent topics of viral infection and cancer therapies using biomolecules-MNCs are addressed.

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