Specific quantitation by HPLC of protein (lysine) bound glucose in human serum albumin and other glycosylated proteins.

Abstract

A specific and sensitive method for quantification of the fructose-lysine linkages present in non-enzymatically glycosylated albumin and other proteins is described. Protein is hydrolyzed for 18 h in 6 mol/l HCl at 95 degrees C to yield furosine (epsilon-N-(2-furoylmethyl)-L-lysine) known as a specific degradation product of fructose-lysine. Furosine is… (More)

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@article{Schleicher1981SpecificQB, title={Specific quantitation by HPLC of protein (lysine) bound glucose in human serum albumin and other glycosylated proteins.}, author={Erwin D. Schleicher and O. H. Wieland}, journal={Journal of clinical chemistry and clinical biochemistry. Zeitschrift fur klinische Chemie und klinische Biochemie}, year={1981}, volume={19 2}, pages={81-7} }