Specific quantification of trichoderma reesei cellulases in reconstituted mixtures and its application to cellulase-cellulose binding studies.

@article{Nidetzky1994SpecificQO,
  title={Specific quantification of trichoderma reesei cellulases in reconstituted mixtures and its application to cellulase-cellulose binding studies.},
  author={Bernd Nidetzky and Marc Claeyssens},
  journal={Biotechnology and bioengineering},
  year={1994},
  volume={44 8},
  pages={961-6}
}
Specific quantifications of the major cellulolytic components of the Trichoderma reesei enzyme complex, i.e., endoglucanases I and III and cellobiohydrolases I and II, are described and, employing a defined mixture of these four cellulases reconstituted according to the composition of the native Trichoderma cellulase complex, used to determine the binding of each individual component onto filter paper. During substrate degradation by this enzyme mixture, the specific adsorption of each… CONTINUE READING

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