Specific properties of enteropathogenic Escherichia coli isolates from diarrheal patients and comparison to strains from foods and fecal specimens from cattle, swine, and healthy carriers in Osaka City, Japan.

@article{Wang2013SpecificPO,
  title={Specific properties of enteropathogenic Escherichia coli isolates from diarrheal patients and comparison to strains from foods and fecal specimens from cattle, swine, and healthy carriers in Osaka City, Japan.},
  author={Li-li Wang and Mitsuko Wakushima and Tetsu Aota and Yuka Yoshida and Toshimasa Kita and Tomofumi 5 Maehara and Jun Ogasawara and Changsun Choi and Yoichi Kamata and Yukiko Hara-Kudo and Yoshikazu Nishikawa},
  journal={Applied and environmental microbiology},
  year={2013},
  volume={79 4},
  pages={1232-40}
}
For exhaustive detection of diarrheagenic Escherichia coli, we previously developed a colony-hybridization method using hydrophobic grid-membrane filters in combination with multiplex real-time PCR. To assess the role of domestic animals as the source of atypical enteropathogenic E. coli (aEPEC), a total of 679 samples (333 from foods, fecal samples from 227 domestic animals, and 119 from healthy people) were examined. Combining 48 strains previously isolated from patients and carriers, 159… CONTINUE READING