Results Median age was 122 months (108 to 143 months) in controls and 142 months (117 to 156 months) in allergic rhinitis group. At the end of the NPT, the mean V5 fall was 5.7% (-8.7% to 4%) in controls and 22.8% (-24% to -20%) in allergic rhinitis group. None of the 15 controls and 88% (15 of 17 patients) of the allergic rhinitis group had a positive Dp NPT. Considering positive NPTs, 23.5% (4/15) were positive at 1:10000, 35% (6/15) at 1:1000, 23.5% (4/15) at 1:100 and 6% (1/15) at 1:10 concentration. One NPT was considered positive due to symptom score. None of the 32 patients presented bronchial reactions or any pulmonary symptom after NPT. Conclusions This protocol has showed to have good specificity and sensitivity to discriminate patients with allergic rhinitis from controls. A simplified protocol with two Dp concentrations (1:1000 and 1:100) seems to be less expensive and less time consuming NPT protocol to be applied in the clinical practice.