Specific knockdown of Oct4 and beta2-microglobulin expression by RNA interference in human embryonic stem cells and embryonic carcinoma cells.

@article{Matin2004SpecificKO,
  title={Specific knockdown of Oct4 and beta2-microglobulin expression by RNA interference in human embryonic stem cells and embryonic carcinoma cells.},
  author={Maryam M Matin and James R. Walsh and Paul Gokhale and Jonathan Draper and Ahmad Reza Bahrami and IanK. M. Morton and Harry D. M. Moore and Peter W. Andrews},
  journal={Stem cells},
  year={2004},
  volume={22 5},
  pages={
          659-68
        }
}
We have used RNA interference (RNAi) to downregulate beta2-microglobulin and Oct4 in human embryonal carcinoma (hEC) cells and embryonic stem (hES) cells, demonstrating that RNAi is an effective tool for regulating specific gene activity in these human stem cells. The knockdown of Oct4 but not beta2-microglobulin expression in both EC and ES cells resulted in their differentiation, as indicated by a marked change in morphology, growth rate, and surface antigen phenotype, with respect to SSEA1… CONTINUE READING

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