Specific inhibition of mature fungal serine proteinases and metalloproteinases by their propeptides.

@article{Markaryan1996SpecificIO,
  title={Specific inhibition of mature fungal serine proteinases and metalloproteinases by their propeptides.},
  author={A. Yu. Markaryan and Joo Dong Lee and Tatiana D. Sirakova and Pappachan Ettoop Kolattukudy},
  journal={Journal of bacteriology},
  year={1996},
  volume={178 8},
  pages={
          2211-5
        }
}
The function of the long propeptides of fungal proteinases is not known. Aspergillus fumigatus produces a 33-kDa serine proteinase of the subtilisin family and a 42-kDa metalloproteinase of the thermolysin family. These extracellular enzymes are synthesized as preproenzymes containing large amino-terminal propeptides. Recombinant propeptides were produced in Escherichia coli as soluble fusion proteins with glutathione S-transferase or thioredoxin and purified by affinity chromatography. A… CONTINUE READING
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