Specific in situ hepatitis B viral double mutation (HBVDM) detection in urine with 60 copies ml(-1) analytical sensitivity in a background of 250-fold wild type without DNA isolation and amplification.

  title={Specific in situ hepatitis B viral double mutation (HBVDM) detection in urine with 60 copies ml(-1) analytical sensitivity in a background of 250-fold wild type without DNA isolation and amplification.},
  author={Ceyhun E. Kirimli and Wei-Heng Shih and Wan Y. Shih},
  journal={The Analyst},
  volume={140 5},
We have examined in situ detection of hepatitis B virus 1762T/1764A double mutation (HBVDM) in urine using a (Pb(Mg(1/3)Nb(2/3))O3)(0.65)(PbTiO3)(0.35) (PMN-PT) piezoelectric plate sensor (PEPS) coated with a 16-nucleotide (nt) probe DNA (pDNA) complementary to the HBVDM. The in situ mutation (MT) detection was carried out in a flow with the PEPS vertically situated at the center of the flow in a background of wild type (WT). For validation, this detection was followed by detection in the… 
Amplification-free in situ KRAS point mutation detection at 60 copies per mL in urine in a background of 1000-fold wild type.
Under such conditions, PEPS was shown to specifically detect KRAS MT in situ with 60 copies per mL analytical sensitivity in a background of clinically-relevant 1000-fold more WT in 30 min without DNA isolation, amplification, or labeling.
Piezoelectric Plate Sensor (PEPS) for Analysis of Specific KRAS Point Mutations at Low Copy Number in Urine Without DNA Isolation or Amplification.
Under these conditions PEPS was shown to specifically detect KRAS MT in situ within 30 min with an analytical sensitivity of 60 copies/mL in a clinically relevant background of WT with concentrations 1000-fold greater than that of MT without DNA isolation, amplification, or labeling.
Rapid, label-free genetic detection of enteropathogens in stool without genetic isolation or amplification.
Ultra-specific nucleic acid testing by target-activated nucleases.
This review focuses on the latest progress in nucleic acid testing methods based on the target-activated nucleases and summarizes the property of enzymes such as CRISPR/Cas, Argonautes, and some gene-editing irrelevant nucleases, which have been leveraged to create highly specific and sensitive nucleic acids testing tools.
A Review of Piezoelectric and Magnetostrictive Biosensor Materials for Detection of COVID‐19 and Other Viruses
The state of the art of research on biosensor materials for virus detection is reviewed and progress related to early detection of COVID‐19 (coronavirus disease 2019) is discussed, where remaining challenges in the field are identified.
An electrochemical sensor based on iron(II,III)@graphene oxide@molecularly imprinted polymer nanoparticles for interleukin-8 detection in saliva
A novel electrochemical sensor for interleukin-8 (IL-8) detection based on Fe3O4@graphene oxide (GO)@molecularly imprinted polymer (MIP) nanoparticles was constructed. IL-8 was surface imprinted on


Temperature- and flow-enhanced detection specificity of mutated DNA against the wild type with reporter microspheres.
It was shown that at room temperature, flow initially increased the binding of both the MT and WT at lower flow rates but decreased the binding at flow rates ≥4 ml min(-1) due to the increase in the flow-induced impingement force on the FRMs to overcome the bound pDNA and the WT to the GCG at higher flow rates.
Specific mutations of hepatitis B virus in plasma predict liver cancer development.
It is found that a prediagnosis biomarker of specific HBV mutations can be measured in plasma and suggested for use as an intermediate endpoint in prevention and intervention trials.
Predictive power of hepatitis B 1762T/1764A mutations in plasma for hepatocellular carcinoma risk in Qidong, China.
Individuals positive for the HBV 1762(T)/1764(A) mutation at enrollment were substantially more probably to subsequently develop HCC, with a higher concentration of the mutation in plasma enhancing predisposition for cancer development.
DNA melting analysis for detection of single nucleotide polymorphisms.
DNA melting analysis (DM) was used successfully for variant detection, and two previously unknown SNPs were discovered by this approach, making it highly suitable for the large-scale detection of sequence variants.
DNA hybridization detection with 100 zM sensitivity using piezoelectric plate sensors with an improved noise-reduction algorithm.
Real-time, in situ hybridization detection of target DNA (tDNA) in a buffer solution and in urine using 8 μm-thick lead magnesium niobate-lead titanate (PMN-PT) piezoelectric plate sensors (PEPSs) and a new multiple-parabola (>50) resonance peak position fitting algorithm is examined.
Transrenal DNA as a Diagnostic Tool: Important Technical Notes
The sensitivity and specificity of detection of mutant sequences are reduced in the presence of high excess of a respective wild‐type allele, but they can be significantly increased through application of enriched polymerase chain reaction (PCR), peptide nucleic acid (PNA) clamped PCR, and/or stencil‐aided mutation analysis (SAMA), based on selective pre‐PCR elimination of wild‐ type sequences.