Specific immobilization of an enzyme by monoclonal antibody: immobilization of guinea pig liver transglutaminase.

@article{Ikura1984SpecificIO,
  title={Specific immobilization of an enzyme by monoclonal antibody: immobilization of guinea pig liver transglutaminase.},
  author={Koji Ikura and Kazuo Okumura and Masakazu Yoshikawa and Ryuzo Sasaki and Hirofumi Chiba},
  journal={Journal of applied biochemistry},
  year={1984},
  volume={6 4},
  pages={222-31}
}
A simple method for enzyme immobilization using a monoclonal antibody raised against the enzyme was studied, taking guinea pig liver transglutaminase as an example. This method consists of two steps; a conjugation of monoclonal antibodies, which bind with the enzyme without inhibitory effect on the activity, with agarose gel bead supports, and immobilization of enzyme proteins onto the conjugated monoclonal antibodies through the antigen-antibody immunoreaction. The following are the advantages… CONTINUE READING

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