Specific gene suppression by engineered ribozymes in monkey cells.

  title={Specific gene suppression by engineered ribozymes in monkey cells.},
  author={Frank Cameron and Philip A Jennings},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  volume={86 23},
Short catalytic RNAs possessing specific endoribonuclease activity (ribozymes) have recently been designed that can potentially shear any chosen target RNA in trans at a specific site. Here, engineered ribozymes targeted against chloramphenicol acetyltransferase (CAT), derived from Tn9, have been cloned into a mammalian expression vector and tested in transient transfection experiments for their effects on CAT expression in monkey (COS1) cells. The ribozymes contained the catalytic domain of… CONTINUE READING