Specific gene silencing using small interfering RNAs in fish embryos.

@article{Boonanuntanasarn2003SpecificGS,
  title={Specific gene silencing using small interfering RNAs in fish embryos.},
  author={Surintorn Boonanuntanasarn and Goro Yoshizaki and Toshio Takeuchi},
  journal={Biochemical and biophysical research communications},
  year={2003},
  volume={310 4},
  pages={
          1089-95
        }
}
Recently, small interfering RNAs (siRNAs) have been used for gene knockdown in mammalian cultured cells, but their utility in fish has remained unexplored. Here we demonstrate a siRNA-mediated gene silencing technique in rainbow trout embryos. We found that siRNAs effectively suppressed the transient expression of episomally located foreign GFP genes at an early developmental stage and inhibited the expression of GFP genes in stable transgenic trout embryos. Similar gene silencing was observed… CONTINUE READING

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